Confidence: Annotation confidence: high Feedback: Do you believe this miRNA is real? Comments: Human miR-92a (previously named miR-92 here) has two predicted hairpin precursor sequences: mir-92a-1 on chromosome 13 (named mir-92-13 in [1]) and mir-92a-2 on chromosome X (named mir-92-X in [1]).miR-92a has also been cloned from mouse embryonic stem cells [2] and is predicted to be …

PCa exosomes and the transferred miRNA, miR‐92a‐1‐5p, are critical mediators that disrupt bone homeostasis, degrade the bone matrix, induce pathological bone remodeling, and, ultimately, create an osteoclastic premetastatic niche for tumor growth. ACKNOWLEDGEMENTS.

mir 92-1, Mirn92-1, mmu-mir-92-1, mmu-mir-92a-1. Feature Type. miRNA gene. IDs. MGI:3629918.

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We found all three miRNAs were universally expressed, but at different level, in the three PCa cell lines (Figure S7a‐c ). miR-92a is reported as an atheroprone miR involved in inflammasome activation in ECs. 21 To explore whether endothelial miR-92a is the mediator for atherogenic EC-induced macrophage inflammation, we investigated miR-92a biogenesis (primary miR [pri-miR]-92a and mature miR-92a) in cocultured ECs and macrophages. 2020-12-10 · Human miRNA expression analysis of chronic hepatitis liver tissue and exosomes, 191-5p, 192-5p, 21a-5p, 22-3p, 26a-5p, and 92a-3p was significantly increased in STZ-mice, Human miR-92a (previously named miR-92 here) has two predicted hairpin precursor sequences: mir-92a-1 on chromosome 13 (named mir-92-13 in [1]) and mir-92a-2 on chromosome X (named mir-92-X in [1]). miR-92a has also been cloned from mouse embryonic stem cells [2] and is predicted to be expressed from two closely related precursor hairpins ( MI0000719 and MI0000580 ). When measuring miR-92a expression in isolated cells or ECV in this study, significant effects on miR-92a expression levels were observed only in the higher dose groups of >0.9 mg/kg, which may suggest measuring of the targeted miRNA by PCR in whole blood overestimates the biological effects due to possible interference of the remaining anti-miR in the sample.

miRNA-92a mimics transfection was detected at 24 h after transfection, then gradually decreased from this level (Fig. 2A) . The miRNA -92a expression levels were reduced in comparison with the NC group 24 h after miRNA-92a inhibitor transfec-

When measuring miR-92a expression in isolated cells or ECV in this study, significant effects on miR-92a expression levels were observed only in the higher dose groups of >0.9 mg/kg, which may suggest measuring of the targeted miRNA by PCR in whole blood overestimates the biological effects due to possible interference of the remaining anti-miR in the sample. miRNA-92a mimics transfection was detected at 24 h after transfection, then gradually decreased from this level (Fig. 2A) .

We suspect that the interactions between miR-92a and ΔSP1 may result from (i) possible G-U wobble base-pairings between the mutated nucleotides and miR-92a; and (ii) extensive base-pairing in the non-seed region of miR-92a (Fig. 1), because the non-seed region of miRNAs (nucleotides 12–17) have been shown in mammalian cells to be important for miRNA targeting [22, 23].

1), because the non-seed region of miRNAs (nucleotides 12–17) have been shown in mammalian cells to be important for miRNA targeting [22, 23]. Nrf1 was predicted to be and confirmed as a potential target for miRNA (miR)-92a-3p. In addition, the inhibition of miR-92a-3p alleviated oxidative stress in vitro and decreased the expression levels of NLRP3, caspase-1,GSDMD-N, IL-1β, and IL-18 in vitro and in vivo. Our results suggest that exosomal miR-92a-3p regulates cartilage development and homeostasis by directly targeting WNT5A. This indicates that exosomal miR-92a-3p may act as a Wnt inhibitor and exhibits potential as a disease-modifying osteoarthritis drug. Serum miRNA levels have attracted attention for their usefulness in the diagnosis and follow-up of tumors. We expected that serum miRNA could also be useful as a marker in collagen disease and that serum miR-92a levels are decreased in SSc patients because miR-92a is a putative down-regulator of integrin, which is up-regulated in SSc. Suppression of miR-92a-1-5p and restoration of FOXD1 may be a preventive approach for gastric IM in patients with bile regurgitation.

Biological Function of the miR-17-92 Cluster.
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Boxplot showing the relative expression distribution of miR-21, miR-31, miR-92a, miR-101, miR-106a and miR-145. qRT-PCR was performed and Ct values for each miRNA was normalized against RNU44 and the relative expression was calculated using 2-dCt method. MicroRNA-92a controls angiogenesis and functional recovery of ischemic tissues in mice.

20,21 This process resulted in 6 candidate miRNAs (miR-15b, miR-19b, miR-21, miR-92a, miR-106b, miR-204) for further investigation. 2020-12-10 Changes in miRNA(s) profile of BeWo cells treated with forskolin were analyzed using Affymetrix miRNA microarray platform. Down‐regulated miRNA, miR‐92a‐1‐5p, was overexpressed in BeWo cells followed by forskolin treatment to understand its relevance in the process of BeWo cell fusion by desmoplakin I+II staining and hCG secretion by ELISA.
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Firstly, we performed qPCR to examine the expression of miRNA‐92a‐1‐5p, miRNA‐375, miR‐148a‐3p in different PCa cells. We found all three miRNAs were universally expressed, but at different level, in the three PCa cell lines (Figure S7a‐c ).

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28 feb. 2021 — Diagram över mikroRNA (miRNA) -åtgärd med mRNA 370 och 381 som reglerar Bcl2L11 (Bim) och miRs ‐ 15b, 18a, 21, 30c, 92a, 106a, 

NanoString nCounter assay The NanoString nCounter Human v3 miRNA Expression Assay has been used to perform the microRNA profiling analysis on RNA samples isolated from PB and PBVs of liposarcoma patients and healthy individual controls. A specific miRNA can be silenced by a complementary antagomir. microRNA maturation can be inhibited at several points by steric-blocking oligos. The miRNA target site of an mRNA transcript can also be blocked by a steric-blocking oligo. For the "in situ" detection of miRNA, LNA or Morpholino probes can be used. 2021-04-06 · The data in the present study demonstrate that inhibition of miR-92a by an LNA-based miRNA inhibitor improves recovery after ischemia/reperfusion in a pig model. In addition, this study provides first evidence that local catheter-based delivery of the LNA-based miR-92a inhibitor more effectively augments the cardioprotective effects than systemic application.

For the "in situ" detection of miRNA, LNA or Morpholino probes can be used.